Response of Dormancy-Capable Breast Cancer D2.0R Cells to Co-Culture with Macrophages
Location
Student Center
Document Type
Poster
Start Date
27-8-2026 11:40 AM
End Date
27-8-2026 12:40 PM
Description
ER+ breast cancer has a 20% recurrence rate in the 15 years following diagnosis. A potential contributor is the dissemination and metastasis of tumor cells to distant sites, where the cells can remain dormant. The tumor microenvironment of breast cancer recruits macrophages that increase tumor proliferation. Based on this behavior at the primary tumor site, it was postulated that macrophages may also be involved in reactivation of dormant cells in distant sites. Specifically, we hypothesized that the interaction between macrophages and dormancy-capable breast cancer D2.0R cells under semi-3D conditions could result in dormancy reversion. A seven-day co-culture of D2.0R cells and J774A.1 macrophages following the standard dormancy Matrigel-on-Top assay was performed, and the proliferation of the D2.0R cells was monitored with Cytation 5 imaging. The primary finding was that the D2.0R cells co-cultured with J774s had 6-fold higher proliferation compared to D2.0Rs cultured alone.
Response of Dormancy-Capable Breast Cancer D2.0R Cells to Co-Culture with Macrophages
Student Center
ER+ breast cancer has a 20% recurrence rate in the 15 years following diagnosis. A potential contributor is the dissemination and metastasis of tumor cells to distant sites, where the cells can remain dormant. The tumor microenvironment of breast cancer recruits macrophages that increase tumor proliferation. Based on this behavior at the primary tumor site, it was postulated that macrophages may also be involved in reactivation of dormant cells in distant sites. Specifically, we hypothesized that the interaction between macrophages and dormancy-capable breast cancer D2.0R cells under semi-3D conditions could result in dormancy reversion. A seven-day co-culture of D2.0R cells and J774A.1 macrophages following the standard dormancy Matrigel-on-Top assay was performed, and the proliferation of the D2.0R cells was monitored with Cytation 5 imaging. The primary finding was that the D2.0R cells co-cultured with J774s had 6-fold higher proliferation compared to D2.0Rs cultured alone.