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Publication Date

2001

Keywords

Wound healing; Myoblasts; Rats

Abstract

Interstitial fluids from late wound repair environments are not "regeneration permissive" as judged by their inability to promote either proliferation or differentiation of myoblasts in vitro. This irwestigation considered whether apoptosis (induced death) was an alternative fate for myoblasts exposed to these interstitial fluids. Myoblast fate was assessed by cell counts, tritiated thymidine release, and propidium iodide staining. Fluids from early wounds increased mean cell counts and induced little thymidine release or propidium iodide labeling. In contrast, fluids from late wounds reduced cell counts and induced both thymidine release and propidium iodide labeling. These data suggest that interstitial fluids from late wounds might trigger apoptosis. It is presently unclear how death is induced in these environments. Further study is required to ascertain whether these effects represent an impediment to skeletal muscle regeneration only or if they reflect a previously unidentified role for late wound fluids in postinjury resolution.

First Page

47

Last Page

53

Included in

Biology Commons

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