Document Type


Publication Date



Wound healing; Myoblasts; Rats


Interstitial fluids from late wound repair environments are not "regeneration permissive" as judged by their inability to promote either proliferation or differentiation of myoblasts in vitro. This irwestigation considered whether apoptosis (induced death) was an alternative fate for myoblasts exposed to these interstitial fluids. Myoblast fate was assessed by cell counts, tritiated thymidine release, and propidium iodide staining. Fluids from early wounds increased mean cell counts and induced little thymidine release or propidium iodide labeling. In contrast, fluids from late wounds reduced cell counts and induced both thymidine release and propidium iodide labeling. These data suggest that interstitial fluids from late wounds might trigger apoptosis. It is presently unclear how death is induced in these environments. Further study is required to ascertain whether these effects represent an impediment to skeletal muscle regeneration only or if they reflect a previously unidentified role for late wound fluids in postinjury resolution.

First Page


Last Page


Primo Type


Included in

Biology Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.