Carrots; Cell division; Plant micropropagation; Plant tissue culture
The intent of this project was to induce the totipotency of carrot cells in tissue culture. The information gathered would be applied to work with woody plants. Differentiated cells removed from the root were cultured in vitro using a modified White's medium. Callus was initiated and successfully sub cultured. Roots and the production of chlorophyll have been initiated in the cultures. Because some specialized equipment and facilities for tissue culture were not available at the beginning of the project, it took considerable time to gather and improvise them. A roller tube culture mixer was constructed, using the mechanism from a strip-chart recorder. Complete regeneration of the whole plant has not been accomplished, but the tissue and organ formation described indicates that totipotency has been stimulated . It shows that successful plant tissue culture studies may be performed in the absence of highly specialized facilities.
Ruser, K. L.
In Vitro Experiments on Totipotency of Dacus Carota.
Journal of the Minnesota Academy of Science, Vol. 45 No.2, 6-7.
Retrieved from https://digitalcommons.morris.umn.edu/jmas/vol45/iss2/3