Document Type


Publication Date



Carrots; Cell division; Plant micropropagation; Plant tissue culture


The intent of this project was to induce the totipotency of carrot cells in tissue culture. The information gathered would be applied to work with woody plants. Differentiated cells removed from the root were cultured in vitro using a modified White's medium. Callus was initiated and successfully sub cultured. Roots and the production of chlorophyll have been initiated in the cultures. Because some specialized equipment and facilities for tissue culture were not available at the beginning of the project, it took considerable time to gather and improvise them. A roller tube culture mixer was constructed, using the mechanism from a strip-chart recorder. Complete regeneration of the whole plant has not been accomplished, but the tissue and organ formation described indicates that totipotency has been stimulated . It shows that successful plant tissue culture studies may be performed in the absence of highly specialized facilities.

First Page


Last Page


Primo Type


Included in

Plant Biology Commons



To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.