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Trematoda; Polyacrylamide gel electrophoresis; Proteins--Structure


Whole-worm extracts of Clinostomum marginatum were subjected to polyacrylamide gel electrophoresis. Gels of early stage metacercariae, late-stage metacercariae and adult worms contained 26,24, and 27 protein bands respectively. The protein band patterns of the three stages were similar except for 1) a hemoglobin band in gels of adult extracts and 2) a large increase in the quantity of four anodic bands of late-stage gels. The proteins constituting these four bands were present in the caecal contents of late-stage metacercariae . These same proteins were also found in metacercarial cysts as part of an exuded material left behind by the fluke after excystment.

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