Mayflies; Cholinesterase-inhibiting insecticides; Solubilization
A method hos been developed for the solubilization of cholinesterase from whole mayflies (Hexagenia bilineata, Soy). Solubilization is enhanced through the use of: (1) protamine sulfate, (2) sephadex, (3) sodium taurocholate, and (4) ammonium sulfate-sodium bicarbonate treatments. Partial purification of the enzyme is also reported. Fractions representing a final activity of 246 units and a purification factor of 17.79 are presented.
Lidgerding, B. C.,
Kraft, D. J.
Mayfly Cholinesterase: Solubilization and Partial Purification.
Journal of the Minnesota Academy of Science, Vol. 36 No.2, 77-79.
Retrieved from https://digitalcommons.morris.umn.edu/jmas/vol36/iss2/6