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Liverworts; Marchantia; Electrophoresis; Proteins--Separation


Extracts from Marchanlia polymorpha were used in the development of a disc electrophoretic procedure for the separation of proteins. Dialyzed and fresh extracts were found to produce best resolution when electrophoresed in Tris-Glycine buffer (pH 8.3) and 50 millamps current for 45-60 minutes. The esterase enzyme system was used as an indication of separation. Significantly, 5 bands were detected in induced thalli attesting to the sensitivity and application of this method to developmental studies.

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